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Disclosed are improved VP2-modified recombinant adeno-associated viral (r AAV) vectors, expression systems, and r AAV virions that are fully virulent, yet lack functional VP2 protein expression. Methods are provided for preparing and using these modified r AAV-based vector constructs in a variety of viral-based gene therapies, and in particular, in the treatment, amelioration, and/or prevention of human diseases.1.2 DESCRIPTION OF RELATED ARTMajor advances in the field of gene therapy have been achieved by using viruses to deliver therapeutic genetic material.Also disclosed are pharmaceutical compositions, virus particles, host cells, and pharmaceutical formulations that comprise these modified vectors useful in the expression of therapeutic proteins, polypeptides, peptides, antisense oligonucleotides and/or ribozymes in the cells and tissues of selected mammals, including, for example, human tissues and host cells. I~I~CI~GIIOUND OF THE INVENTIONThe present application claims priority to l Tnited States Provisional Application Serial No. The adeno-associated virus (AAV) has attracted considerable attention as a highly effective viral vector for gene therapy due to its low immunogenicity and ability to effectively transduce non-dividing cells.We have engine parts for Briggs and Stratton, Tecumseh, Kohler, Honda, Kawasaki.Mower Parts also carries a complete line of go-kart parts including roller chain, clutches, brake bands, engine parts, tires and more!All of these constructs—intimacy, relational closeness, bondedness, attachment, and involvement—can be conceptualized as multicomponential constructs that include or are relevant to interpersonal warmth.Close relationships are affectively intense with positive emotions typically dominating.The natural tropism of AAV for the abundantly expressed HSPG presents a challenge to specifically targeting particular cell populations.
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Such plasmids can be complemented with plasmids that express the remaining 2 capsid proteins (p IM45-VP2,3, p IM45-VP1,3, and p IM45-VP1,2, respectively) in order to produce viable recombinant AAV2 vectors.
Interestingly, the plasmid, p IM45-VP1,3 is also capable of producing infectious virions in the absence of VP2 expression.
The invention provides VP2-modified recombinant adeno-associated virus (r AAV) vectors that, while deleted for VP2, are still fully virulent.
'The United States government has eertain rights in the present invention pursuant to grant numbers P50 HIJ59412, P~1 HI,51S11 and T32 AI 7110 from the l~Iational Institutes of Health.1.1 FIELD OF THE INVENTIONThe present invention relates generally to the fields of molecular biology and virology, and in particular, to the development of gene delivery vehicles.